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Non-coding RNA Research

A closed-loop functional and mechanistic research path for non-coding RNAs such as miRNA, lncRNA, and circRNA.

TECHNICAL ROUTE

Technical Route

1 Construct Functional Vector Overexpression | Sponge (Knockdown) Vector 2 Target Gene Prediction & Validation Dual-Luciferase Binding Validation 3 ceRNA Mechanism Research miRNA Sponge Assay 4 Cellular Function Validation Phenotype-Level Functional Confirmation
RESEARCH STEPS

Research Steps & Corresponding Services

1

Construct a Functional Vector

Build an miRNA overexpression/knockdown (Sponge) vector via molecular cloning, and deliver it into cells or an animal model via viral packaging.

Molecular Cloning / Subcloning →Viral Packaging Services →
2

Target Gene Prediction & Validation

Use a dual-luciferase reporter assay to validate the binding relationship between the non-coding RNA and the predicted target gene's 3'UTR.

Dual-Luciferase Reporter Assay →
3

ceRNA Mechanism Research

Study the molecular mechanism within the competing endogenous RNA network via an miRNA sponge assay.

4

Cellular Function Validation

Confirm the non-coding RNA's biological function at the phenotypic level via cell function assays.

Cell Function Assays →

Need a Plan Tailored to Your Project?

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OTHER SOLUTIONS

Other Solutions

Gene Function Research
Molecular Mechanism Research
Molecular Interaction Research
Expression Regulation Research
Non-coding RNA Research
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