First confirm expression changes in key candidate-pathway molecules with qPCR and Western Blot, to lock in the research direction.
Use a dual-luciferase reporter assay to detect the transcriptional activity of the pathway's downstream reporter gene, determining whether the pathway is activated.
Obtain a whole-transcript expression profile via RNA-seq, then run GO/KEGG enrichment through bioinformatics analysis to unbiasedly screen the affected pathway network.
Run a rescue experiment via viral packaging or gene editing to reverse-validate the causal relationship between the pathway and the phenotype, closing the logical loop.
Submit your research background, and a technical advisor will help identify the best research path and service combination for you.
Submit Your Project