We offer custom recombinant protein services across multiple expression systems — E. coli, yeast, mammalian cells (HEK293/CHO), and insect-baculovirus — covering expression vector construction, small-scale expression optimization, large-scale fermentation/culture, purification, and QC.
When you need a high-purity target protein for antibody development, structural research, or functional experiments, and the protein may require complex post-translational modifications (such as glycosylation), choosing the right expression system ensures you get an active, functional protein — answering "how do you actually get a protein that ‘works.’"
The gene of interest is cloned into an expression vector containing an inducible promoter and transformed/transfected into a host cell; the target protein is expressed after induction, then purified through multiple steps such as tag-affinity chromatography (e.g., Ni-NTA) combined with ion exchange and size-exclusion chromatography to obtain a high-purity protein. Eukaryotic systems are suited to complex proteins requiring post-translational modifications such as glycosylation.
Antigen preparation, enzyme activity research, structural analysis, interaction research, antibody drug development, etc.
Prokaryotic, yeast, mammalian, or insect cells, as needed
Specific timeline assessed by protein difficulty
His/GST/Fc and other tags, tagged or tag-free
Delivered with SDS-PAGE, concentration, and purity reports
Submit your experimental plan and objectives
Technical advisor confirms the protocol and timeline
Pay a deposit of at least 50% of the project cost to enter production
Quality control documented throughout production
Settle the balance and complete acceptance
Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.
Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.