A one-stop service from sample RNA/DNA extraction and reverse transcription to qPCR detection, supporting differential gene expression analysis, miRNA expression detection, gene copy number determination, and knockdown efficiency assessment, with both SYBR Green and TaqMan probe options.
When you need to know exactly how much the expression of a gene/miRNA has changed — not just whether it changed — qPCR is the most commonly used quantitative verification method — answering "how large is the change in expression, and is it statistically significant."
A fluorophore is added to the PCR system, and the fluorescence signal from the amplified product is monitored in real time at each cycle to quantify the starting template. Supports absolute quantification (standard curve method) and relative quantification (normalized to a reference gene, commonly using the 2⁻ΔΔCt method).
Gene expression verification, interference/overexpression efficiency verification, copy number analysis, pathogen detection, etc. — one of the most widely used molecular verification methods in gene function and molecular mechanism research.
SYBR Green for low-cost speed, or TaqMan for high-specificity multiplexing
Predictable standard turnaround time
Complete report with amplification curves, melt curves, and differential analysis
Standard curve method or 2⁻ΔΔCt method, matched to your needs
Submit your experimental plan and objectives
Technical advisor confirms the protocol and timeline
Pay a deposit of at least 50% of the project cost to enter production
Quality control documented throughout production
Settle the balance and complete acceptance
Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.
Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.