Bacterial Strains

Strains (Competent Cells) 358 菌株 283

Escherichia coli 424 Saccharomyces cerevisiae 61 Agrobacterium tumefaciens 37 Agrobacterium rhizogenes 25 Pichia pastoris 25 Bacillus subtilis 11 Pseudomonas aeruginosa 6 Staphylococcus aureus 5 Aspergillus niger 3 Lactococcus lactis 3 Salmonella 3 Candida albicans 2 Clostridium sporogenes 2 Listeria monocytogenes 2 Shigella flexneri 2 Streptococcus lactis 2 Acinetobacter baumannii 1 Aspergillus brasiliensis 1 Bacillus cereus 1 Bacillus licheniformis 1 Bacillus pumilus 1 Bacillus thuringiensis 1 Bifidobacterium bifidum 1 Caenorhabditis elegans 1 Candidozyma / Candida utilis 1 Clostridium perfringens 1 Corynebacterium glutamicum 1 Cronobacter sakazakii 1 Enterobacter aerogenes 1 Enterococcus faecalis 1 Komagataeibacter xylinus 1 Listeria innocua 1 Listeria ivanovii 1 Micrococcus luteus 1 Pseudomonas putida 1 Pseudomonas syringae 1 Shewanella 1 Shigella dysenteriae 1 Staphylococcus epidermidis 1 Staphylococcus haemolyticus 1 Staphylococcus saprophyticus 1 Streptococcus pyogenes 1 Vibrio parahaemolyticus 1 Yersinia enterocolitica 1

Engineered Strain (Non-wild-type) 573 Standard/Reference Strain (ATCC/CMCC/CICC, etc.) 53 Standard/Reference Strain (Wild-type/Unmodified) 15

recA⁻/endA⁻ Cloning-Stable Strain (reduced homologous recombination and non-specific endonuclease activity; improves plasmid transformation efficiency and stability; for routine plasmid cloning/storage) 139 DE3 Lysogen · T7 Expression Strain (carries T7 RNA polymerase; enables IPTG/T7 promoter-inducible protein expression) 137 No laboratory cloning modification; handled as a standard isolate/reference strain 67 Ti Plasmid Virulence-Gene-Deleted Strain ("disarmed" Agrobacterium tumefaciens; tumor-inducing virulence genes removed while vir-mediated transformation ability is retained; for plant genetic transformation) 38 Auxotrophic · Reporter-Gene Strain (dedicated yeast two-/one-hybrid strain combining auxotrophic markers with reporter genes such as HIS3/LacZ/AUR1-C; for protein-protein/protein-DNA interaction detection) 26 Methanol-Inducible Expression Strain (dedicated host for the Pichia pastoris AOX1 promoter methanol-induction system) 25 Standard Laboratory Background Yeast Strain (auxotrophic markers for plasmid selection, no additional reporter genes; for routine expression/genetic manipulation) 23 Ri Plasmid Hairy-Root-Inducing Strain (Agrobacterium rhizogenes carrying the Ri plasmid; for inducing plant hairy roots/hairy root culture systems) 22 K-12-Derived Wild-Type Background Strain (standard laboratory K-12 lineage without additional cloning-specific mutations; commonly used as a genome-editing/metabolic-engineering chassis) 18 π Protein (pir)-Expressing Strain (for conjugative transfer of R6K-origin suicide plasmids) 16 Enhanced Disulfide-Bond Oxidative Folding Strain (trxB/gor and related mutations promote cytoplasmic disulfide-bond formation; for expressing disulfide-bond-containing proteins) 11 recBC Recombination-Deficient Strain (high recombination efficiency; commonly used for homologous-recombination-based construction of large plasmids such as adenoviral vectors) 10 ccdB Toxin-Gene-Resistant Strain (dedicated to cloning Gateway and other vectors carrying the ccdB lethal gene) 8 DE3 Lysogen · T7 Expression Strain (the T7 Express series is a BL21-derived strain carrying T7 RNA polymerase; for IPTG-inducible protein expression) 8 F' Phagemid Host Strain (carries the F pilus; for M13 phage/phage display experiments) 8 recA⁻/endA⁻ High-Efficiency Cloning Strain (high-efficiency electro-/chemically-competent cells, functionally similar to an enhanced DH5α; for routine plasmid cloning) 7 Protease-Deficient · Recombinant Protein Expression Strain (lon/ompT and related protease deficiencies reduce recombinant protein degradation; for inducible protein expression) 7 Single-Gene-Knockout Background Strain (standard background strain from the Saccharomyces Genome Deletion Project; parental background for single-gene knockout libraries) 6 Rare-Codon-Compensating Strain (carries a tRNA-supplementing plasmid to improve expression of genes with rare codons; often combined with DE3) 5 Restriction-Modification-Deficient Classic Cloning Host (hsdR/mcrA/mcrB and related restriction-system deficiencies; for routine λ phage/plasmid propagation; some strains carry conjugative transfer ability) 5