We offer a targeted yeast one-hybrid verification service for studying the binding relationship between a protein (such as a transcription factor) and a specific DNA cis-element — a commonly used tool for screening protein-DNA interactions in transcriptional regulation research.
When you want to know exactly which proteins can bind a given DNA sequence — rather than verifying, in the other direction, the relationship between an already-known protein and a known DNA sequence — yeast one-hybrid helps you screen candidate binding proteins from a protein library — answering "who recognizes this DNA sequence."
A candidate DNA cis-element (bait) is cloned upstream of a reporter gene (such as HIS3 or LacZ) to construct a reporter vector, which is transformed into a yeast strain carrying a prey protein (such as a transcription factor) expression plasmid. If the prey protein binds the DNA element, it recruits the transcription machinery to activate reporter gene expression, and binding is judged by auxotrophic selection or a colorimetric reaction.
Transcription factor binding site verification, promoter/enhancer cis-element functional research, transcriptional regulatory network analysis, etc.
Targeted at transcription factor-cis-element binding verification
Covers vector construction, transformation, and screening
Confirmed by auxotrophic selection or a colorimetric reaction
Positive results can be further confirmed with EMSA/ChIP
Submit your experimental plan and objectives
Technical advisor confirms the protocol and timeline
Pay a deposit of at least 50% of the project cost to enter production
Quality control documented throughout production
Settle the balance and complete acceptance
Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.
Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.