Using fluorescent tag fusion or specific-antibody immunofluorescence staining combined with organelle marker co-localization, we analyze the distribution of a target protein across subcellular structures such as the nucleus, mitochondria, endoplasmic reticulum, and Golgi apparatus.
When you want to know exactly where a protein resides within the cell, in order to infer the biological processes it may be involved in, subcellular localization provides direct spatial evidence — answering "exactly where in the cell does this protein reside."
The target protein is fused to a fluorescent protein (such as GFP) for expression, or an endogenous protein is labeled with a specific primary antibody and fluorescent secondary antibody, then observed under a fluorescence or confocal microscope. Co-staining with known organelle markers (e.g., DAPI for the nucleus, MitoTracker for mitochondria) confirms the localization relationship.
Protein function research, signal transduction mechanism analysis, organelle interaction research, etc.
Fluorescent fusion expression or immunofluorescence staining, either available
Covers labeling, imaging, and analysis
Includes fluorescence images and interpretation of the localization relationship
Nucleus/mitochondria/endoplasmic reticulum/Golgi apparatus, and more
Submit your experimental plan and objectives
Technical advisor confirms the protocol and timeline
Pay a deposit of at least 50% of the project cost to enter production
Quality control documented throughout production
Settle the balance and complete acceptance
Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.
Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.