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Immunohistochemistry (IHC)
In Situ Expression & Localization Detection of Target Proteins

Immunohistochemical staining of paraffin/frozen tissue sections to detect the expression level, distribution, and localization of a target protein in tissue, supporting single and multiplex staining, with optional digital pathology scanning and semi-quantitative analysis.

Purpose & Application Scenarios

Purpose

When you need to observe the expression distribution and intensity of a target protein in tissue sections while preserving tissue morphology, IHC is the most commonly used tool in pathology and basic research — answering "in which regions of the tissue is this protein expressed, and how much."

Application Scenarios

Technical Principle & Applications

Technical Principle

A specific antibody binds the target antigen in the tissue section; an enzyme-labeled (e.g., HRP) secondary antibody then reacts with a chromogenic substrate (e.g., DAB) to form a visible brown precipitate at the site of antigen expression, enabling in situ localization and expression evaluation while preserving tissue morphology.

Applications

Tumor marker detection, tissue protein expression profiling, clinical pathology research, etc.

Service Advantages

Single or Multiplex Staining

Supports combined detection of multiple targets

Typically 2-3 Weeks

Covers staining, scanning, and scoring analysis

Semi-Quantitative Scoring

Digital pathology scan plus a scoring report

Preserves Tissue Morphology

In situ localization and expression evaluation together

Delivery

Immunohistochemical StainingAbout 2-3 weeks
Deliverables: stained sections, scanned images, and a scoring analysis report. We recommend confirming antibody selection with a technical advisor in advance.

Ordering Process

1

Requirement Discussion

Submit your experimental plan and objectives

2

Proposal Confirmation

Technical advisor confirms the protocol and timeline

3

Deposit & Order Placement

Pay a deposit of at least 50% of the project cost to enter production

4

Production & QC

Quality control documented throughout production

5

Delivery & Acceptance

Settle the balance and complete acceptance

Payment & Acceptance Terms

Project Deposit

Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.

Acceptance

Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.

Disclaimer: The turnaround times and technical parameters shown on this page are typical reference ranges; the order confirmation governs the actual terms. ↑ Back to top
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