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Gene Sequencing
First-, Second-, and Third-Generation Sequencing Platforms

Covering first-generation (Sanger), second-generation (NGS), and third-generation long-read sequencing platforms, we provide one-stop services from single-sample verification to high-throughput omics, meeting needs ranging from clone verification to whole-genome analysis.

Purpose & Application Scenarios

Purpose

When you need to confirm exactly what a DNA sequence is, or whether it carries a variant or an error, sequencing is the way to get a final, direct answer — answering "what does this sequence actually look like."

Application Scenarios

Technical Principle & Applications

First-Generation Sequencing (Sanger)

Used for plasmid verification, clone identification, SNP/SSR site confirmation, MNP genotyping, and more — long read length and high accuracy, based on the dideoxy chain-termination method (ddNTP termination + capillary electrophoresis separation).

Second-Generation Sequencing (NGS)

Suited to whole-genome resequencing, transcriptome (RNA-seq), amplicon (16S/ITS), exome, and other high-throughput sequencing needs. Based on sequencing-by-synthesis (SBS), reading short fragments in massive parallel.

Third-Generation Sequencing (Long-Read)

Suited to full-length transcripts, de novo genome assembly, structural variant detection, methylation detection, and more, with read lengths reaching tens of kb. Based on single-molecule real-time sequencing (SMRT) or nanopore electrical signals.

Applications

Clone verification, population genetics, gene expression profiling, variant detection, microbial diversity, epigenetics, etc.

Service Advantages

Full Coverage Across Three Platforms

Sanger, NGS, or third-generation long-read, as needed

Sanger in 1-2 Business Days

NGS/third-generation assessed by project scale

Raw Data + Analysis Report

Fastq data delivered with a bioinformatics analysis report

Multi-Scenario Fit

From clone verification to variant detection, fully covered

Delivery

Sanger SequencingTypically 1-2 business days
NGS / Third-Generation SequencingTimeline assessed by project scale
Deliverables: Sanger sequencing delivers sequence chromatograms and alignment results; NGS/third-generation sequencing delivers raw data (fastq) and a bioinformatics analysis report.

Ordering Process

1

Requirement Discussion

Submit your experimental plan and objectives

2

Proposal Confirmation

Technical advisor confirms the protocol and timeline

3

Deposit & Order Placement

Pay a deposit of at least 50% of the project cost to enter production

4

Production & QC

Quality control documented throughout production

5

Delivery & Acceptance

Settle the balance and complete acceptance

Payment & Acceptance Terms

Project Deposit

Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.

Acceptance

Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.

Disclaimer: The turnaround times and technical parameters shown on this page are typical reference ranges; the order confirmation governs the actual terms. ↑ Back to top
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