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Genome-Wide / Sub-Pool Library Screening
Genome-Wide · Sub-Pool Libraries

Backed by in-stock CRISPR sgRNA libraries (human, mouse, livestock/poultry, African green monkey, microbial, and other species, with >99% coverage), we offer genome-wide/sub-pool high-throughput screening, custom arrayed libraries, Cas9 stable cell line construction, and one-stop NGS + bioinformatics analysis.

Purpose & Application Scenarios

Purpose

When you’re still not sure "which genes are involved in this phenotype/pathway" and need to screen candidate genes without bias across the whole genome, CRISPR library screening is currently the most mainstream approach — answering "among all these genes, which one is actually responsible."

Application Scenarios

Technical Principle & Applications

Genome-Wide / Sub-Pool Library Screening

Covers 30+ sub-pool libraries for specific pathways such as cell cycle, apoptosis, signal transduction, and the immune system; genome-wide unbiased screening is also available.

Custom Arrayed Libraries

Custom arrayed libraries built to research needs, for well-by-well functional validation.

Cas9 Stable Cell Line Construction

Cas9 stable cell line construction service, providing a qualified chassis cell line for library transduction.

NGS Sequencing & Bioinformatics Analysis

NGS sequencing after library transduction, with enrichment analysis completed using standardized pipelines such as MAGeCKFlute.

Service Advantages

Broad In-Stock Coverage

In-stock libraries for human, mouse, livestock/poultry, African green monkey, microbial, and other species

>99% Coverage

Uniformity <10, reducing downstream screening workload

NGS + MAGeCKFlute

Standardized bioinformatics pipeline with a reproducible analysis report

Puro Selection Stable System

Drug-selectable vector improves transfection/screening efficiency

Delivery

Cas9 Stable Cell Line ConstructionAbout 3-4 weeks (single-clone verification)
Library Transduction + Screening + NGS AnalysisAbout 6-10 weeks (depending on screening scale and complexity)
Deliverables: NGS detection report and a screening enrichment analysis report. Cell quantity, MOI, and other parameters for library transduction will be confirmed by a technical advisor based on the experimental design.

Ordering Process

1

Requirement Discussion

Submit your experimental plan and objectives

2

Proposal Confirmation

Technical advisor confirms the protocol and timeline

3

Deposit & Order Placement

Pay a deposit of at least 50% of the project cost to enter production

4

Production & QC

Quality control documented throughout production

5

Delivery & Acceptance

Settle the balance and complete acceptance

Payment & Acceptance Terms

Project Deposit

Standard, custom, and large orders all require a deposit of at least 50% of the contract value to begin. The timing for the remaining balance is agreed separately for each project.

Acceptance

Please complete acceptance within 1 month of receiving the product or report. Any concerns about the deliverable should be raised within this window; we will respond within 2 business days.

Disclaimer: The turnaround times and technical parameters shown on this page are typical reference ranges; the order confirmation governs the actual terms. ↑ Back to top
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