In-stock reagents for molecular and cell biology work, with stable quality and consistent lot-to-lot performance.
High-efficiency, low-toxicity DNA/RNA transfection reagents suited to a wide range of cell types.
Polymerases, ligases, restriction enzymes, markers, and other core molecular cloning reagents.
Dedicated media such as DMEM and RPMI-1640, with fetal bovine serum (FBS) lot-tested and sample-hold available.
Covering lipid-based and polymer-based transfection methods, suited to both adherent and suspension cell scenarios.
Polymerases, ligases, restriction enzymes, DNA/protein markers, and more, covering the full standard molecular cloning workflow.
Serum for cell culture, lot-tested, with sample-hold confirmation available before large-volume purchase.
Ampicillin, kanamycin, puromycin, G418, and more, for positive clone selection and stable cell line construction.
Lipid-based transfection reagents are simple to use and broadly applicable, making them the default choice for most adherent cell lines. But for hard-to-transfect cell types such as primary or suspension cells, lipid-based efficiency is often disappointing — in those cases, electroporation or virus-mediated delivery (such as lentiviral transduction) tends to work better. Before choosing, it's worth checking whether an established transfection protocol already exists for your target cell type.
Even fetal bovine serum (FBS) lots with identical stated specifications can differ in actual growth-factor content and their ability to support proliferation — an effect that's especially noticeable in serum-sensitive cell lines. A sample-hold confirmation lets you validate that a given lot works with your experimental system on a small scale before committing to a large purchase, avoiding an entire experiment run into unexplained growth abnormalities just because the serum lot changed.
These enzymes typically need to be stored at -20°C and are sensitive to repeated freeze-thaw cycles. It's best to aliquot them into single-use portions rather than repeatedly thawing the main stock, and to work on ice throughout to minimize activity loss.
Sensitivity to the same antibiotic varies widely between cell lines. Before formal selection, it's best to run a kill curve — treating untransfected wild-type cells with a concentration gradient — to find the lowest concentration that kills all cells within a reasonable time, then use that for the actual selection. This avoids failed selection or accidentally killing positive clones from an ill-chosen concentration.