In-stock sgRNA libraries across human, mouse, livestock and poultry, African green monkey, microbial, and other species, with >99% coverage and excellent uniformity to ease downstream screening.
Covering 30+ pathway-specific sub-libraries — cell cycle, apoptosis, signal transduction, immune system, and more — plus unbiased genome-wide screening.
In-stock CRISPRa activation libraries and CRISPRi/CRISPRoff inhibition libraries.
Custom arrayed libraries built to your research needs, for well-by-well functional validation — see the CRISPR Library High-Throughput Screening research service.
Library coverage >99% with excellent sgRNA uniformity (uniformity <10), easing the downstream screening workload.
In-stock sgRNA libraries for human, mouse, livestock and poultry, African green monkey, microbial, and other species.
Library transduction, Cas9 stable cell line construction, NGS sequencing, and bioinformatics analysis — see CRISPR Library High-Throughput Screening.
The in-stock genome-wide/sub-pathway knockout libraries are already-built pooled libraries, ready to use straight out of the box for large-scale screening. If you've already run an initial screen and have candidate genes you need to validate individually, or you need finer single-cell-level phenotype readout, you'll need a custom arrayed library — a custom service, not part of the in-stock inventory.
Coverage (>99%) is the proportion of genes/sites the library design actually reaches — the higher the coverage, the more complete the library's "gene list" and the fewer targets it misses. Uniformity reflects how evenly the abundance of individual sgRNAs is distributed across the library — better uniformity means each sgRNA starts the screen with closer-to-equal representation, reducing statistical noise from a handful of over- or under-represented sgRNAs later on.
In-stock libraries are typically supplied as plasmid-form sgRNA libraries. To use them for cell-based screening, they still need to be packaged into virus (lentivirus) for efficient delivery into cells. This can be handled together with the CRISPR Library High-Throughput Screening research service, or packaged in-house, depending on your lab's setup and needs.
No. CRISPRa (activation)/CRISPRi (interference) libraries use a catalytically dead dCas9 system, and their sgRNA design positions and strategy differ from standard knockout libraries (which rely on active Cas9 creating DNA breaks) — typically targeting regions near a gene's transcription start site. The two are purpose-built for different perturbation mechanisms and aren't interchangeable.